[Macroscopic Solutions Support]

Macroscopic Solutions Support answer
This staff-authored guidance is generalized. Project-specific and private details should be handled through direct support.

The focus increment must leave enough depth-of-field overlap between adjacent frames; higher magnification and higher numerical aperture generally require smaller steps. Settle time must also allow the stage, specimen, cables, and support to stop moving before exposure. Run a short range first, inspect for soft gaps or repeated vibration, and then adjust step size and delay.